ფილმში ასევე მიიღებს მონაწილეობას:

- Andrew Garfield as Peter Parker / Spider-Man
- Emma Stone as Gwen Stacy
- Rhys Ifans as Dr. Curt Connors / The Lixard
- Martin Sheen as Ben Parker
- Sally Field as Mary Parker
- C. Thomas Howell as Ray
- Denis Leary as George Stacy
- Julianne Nicholson as Mary Parker
- Campbell Scott as Richard Parker
- Annie Parisse as Mrs. Van Adder
- Irrfan Khan as Nels Van Adder / Proto-Goblin
- Miles Elliot as Billy Connors
- Chris Zylka
- Charlie DePew as Philip Stacy